The Docklands Light Railway has been served by three different types of rolling stock over the years, two of which are in service. The original P86 and P89 stock was in service from the system opening in 1987 (1989/1990 in the case of the P89 stock) until 1995; they are now in service in Essen, Germany following an extensive rebuild. The B90 and B92 stock entered service in 1992 and 1995, respectively. These cars featured a new interior design to facilitate increased ridership. The newest cars, the B07 Stock, entered service in 2009 in order to facilitate network expansion.

All the rolling stock types are bi-directional, single-articulated electrical multiple units. They typically operate in two or three car sets under automated operation. A staff member is on board who can take over operation if necessary.