Route 99 was a tourist route, making 28 stops at major tourist attractions such as Mount Scopus, the Western Wall, Herzl Museum, Yad Vashem, the Israel Museum, and Knesset, as well as major hotels, on a two hour loop of the city. The buses were equipped with a stereo system and headphones were provided to all riders, allowing them to hear a description of the route in one of eight languages (Hebrew, Arabic, English, French, German, Russian, Italian and Spanish) as they traveled. Tickets for Route 99 were valid all day on the day of use and riders could hop on and hop off the bus as much as they want. Route 99 made 4 complete loops of the city Sunday through Thursday and operated a more limited schedule on Friday.

In 2005, Egged retrofitted several of its retired Neoplan Skyliner double-decker buses to operate on Route 99 in Jerusalem; some of these buses came with open tops for use in the summer while the remainder were closed for winter service. In 2010, a new double decker bus built by MAN with a roof that can be opened like that on a convertible car was delivered and entered service. The Neoplans were placed in storage and used only as backups.

Egged transferred the route to Yara Tours in 2015 and Yara Tours, after operating it as an on demand service, discontinued the route entirely sometime in late 2015 or early 2016. The current status of the MAN Double-Decker that was purchased for use on Route 99 is unknown.